Development of Fe3O4/reduced graphene oxide nanocomposite-based dispersive solid-phase extraction for the quantification of five steroid hormones

Çağatay N. U., Maviş M. E., ERARPAT S., BAKIRDERE S.

Bioanalysis, cilt.15, sa.9, ss.503-512, 2023 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 15 Sayı: 9
  • Basım Tarihi: 2023
  • Doi Numarası: 10.4155/bio-2023-0025
  • Dergi Adı: Bioanalysis
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.503-512
  • Anahtar Kelimeler: dispersive solid-phase extraction, liquid chromatography–tandem mass spectrometry, magnetic nanoparticles, serum samples, steroid hormones
  • Yıldız Teknik Üniversitesi Adresli: Evet

Özet

Aim: An accurate and sensitive analytical method was proposed to detect some steroid hormones in biological samples. Materials & methods: An Fe3O4/reduced graphene oxide nanocomposite-based dispersive solid-phase extraction was developed for the effective and simple preconcentration of steroid hormones from human serum samples. Results & conclusion: The nanocomposite was firstly used as adsorbent to simultaneously extract the selected hormones. Limit of detection values for the selected hormones were calculated between 5.5 and 39.2 ng/kg (mass based). An artificial serum sample was used to test the applicability and accuracy of the developed method; percentage recovery results obtained from two different spiked concentrations were found to be in the range of 80.5-99.9%.