Archaeometric investigations were carried out to determine and to correlate the chemical contents with the layers of deterioration of the 19 glass finds dating to the Roman Period uncovered from Su Terazisi and Kral Kızı Regions of Enez (Ainos) ancient city. The physical condition of glass finds was firstly examined, the layers of deterioration on the glass surface were documented with photographs, their thickness was measured with a digital thickness meter, their colors were identified by chromameter, and images of the bubbles and deterioration products were taken under an optical microscope. XRF (Polarized Energy Dispersive-X-Ray Fluorescence (PED-XRF) and Micro-XRF), Scanning Electron Microscope-Energy Dispersive Spectrometry (SEM-EDS), and Raman (Confocal) Spectroscopy methods were used to determine the element and mineralogical contents, deterioration layer of the glass samples. Glass can undergo chemical reactions in time, due to contact with water and/or humidity within air. Those reactions are highly dependent on the glass composition, its production temperature in kiln and firing duration, moisture, pH and temperature of the medium around glass. When the deterioration layers of the samples are examined visually and with an optical microscope; types of deterioration resulting from the chemical composition of glass, such as iridescence, dulling, black discoloration and pitting have been identified. The gel layer originally formed on the glass surface is rich in silica, and poor in sodium and calcium. The high content of SiO2 in the deterioration layers supports the deterioration in the glasses. It is expected that the amount of flux agent (Na2O) and stabilizer (CaO) in the gel layer of the surface to decrease caused by glass objects contacting with moisture and/ or water. However, the high amount of Na2O and CaO in the results of SEM-EDS analysis of the samples is thought to be due to the accumulation caused by the marine effect as a result of the ancient city being very close to sea. As manganese and iron stand out in black discoloration type deterioration products through analysis of samples, the presence of manganese and iron contents determined by Raman analysis supports these results. Keywords: Archaeological Glass Chemistry, Archaeological Glass Deteriorations, PEDXRF, SEM-EDS, Raman (Confocal) Spectroscopy.