Design and development of a personalized medicine oriented microfluidic organ on a chip platform
Tez Türü: Yüksek Lisans
Tezin Yürütüldüğü Kurum: Gebze Teknik Üniversitesi, Biyoteknoloji Enstitüsü, Biyoteknoloji Anabilim Dalı (Disiplinlerarası), Türkiye
Tez Danışmanı: Ali Akpek
Tezin Onay Tarihi: 2020
Tezin Dili: Türkçe
Özet:
Bringing a drug in health sector costs about 2 billion dollars and the process lasts 12 to 15 years. One of the most important steps of these studies is preclinical testing which is also highly crucial in cosmetic toxicity tests and disease modelling tests. Organs on chip platforms that interconnect bioengineering, chemical engineering and material engineering are the most recent discovery which have demonstrated their capability for drug development, cosmetic toxicity testing, and disease modelling implementation instead of animal models. In this study, PMMA utilized chip was fabricated and GelMA - alginate mixture containing scaffold was fabricated via using a 3D bio-printer and then inserted in the chip system in order to mimic tissue scaffold. Afterwards, mouse fibroblast cells (3T3) were cultured on the chip system, the system was constantly fed with growth culture by using syringe pump. As a control experiment a Petri dish was used and instead of the designed organ on a chip platform. The results were compared with each other. The study was conducted as four different stages: First, an original design for the organs on a chip system was developed and realized. Second, several biomaterials were developed. These biomaterials are 7% - 5% GelMA - alginate mixture, 7% - 4% GelMA - alginate mixture, 5% - 4% GelMA - alginate mixture and 10% - 7% GelMA - alginate mixture. As a third step, these biomaterials were placed to Petri dishes and organs on a chip platforms and fibroblast cells were introduced to both systems. Finally, cell viability results were compared with each other. Results proved that the integrity of the scaffolds were more stable in the chip system and the cell viability was higher compared to Petri dishes. The research indicated an obvious supremacy of organs on chip platforms than 2D platforms thus providing a great promise for reducing the usage of animal in preclinical testing and disease modelling in the years to come. It is proven that in order to achieve more accurate results instead of 2D platforms, 3D structures such organs on a chip platform should be favored. Keywords: microfluidics, organ-on-a-chip, 3D bio printing, disease modelling, animal testing