Akademik Veri Yönetim Sistemi
Tezin Türü: Doktora
Tezin Yürütüldüğü Kurum: Yıldız Teknik Üniversitesi, Fen-Edebiyat Fakültesi, Fizik Bölümü, Türkiye
Tezin Onay Tarihi: 2021
Tezin Dili: Türkçe
Öğrenci: M. Habib ÖNEN
Danışman: Erol Kam
Özet:
Evaluation of Heavy Metal Concentraitons in
Postmortem Tissues Using Laser Induced Breakdown Spectroscopy
Heavy metal intoxication, which is among the major
global problems today due to the rapidly increasing heavy metal pollution since
the industrial revolution, can lead to many health problems and deaths. Elemental
analysis methods as screening and diagnostic tests are needed in the detection
of heavy metal intoxicity and exposure. Laser Induced Breakdown Spectroscopy
(LIBS), as a versatile and multi-elemental technique, has been used for
different aims on organism tissues in the last two decades. In this study; it
was aimed to determine the efficiency of LIBS technique in heavy metal analysis
in postmortem tissues. Cardiac blood was sampled from 67 adult cases autopsied
in Istanbul, liver, kidney, brain, muscle and adipose tissue were sampled 50 of
these cases, and LIBS was applied to the tissues. In addition, by applying
Inductively Coupled Plasma Mass Spectroscopy (ICP-MS), which is a valid
technique in heavy metal analysis, to blood samples; concentrations of mercury,
lead, cadmium, chromium, nickel, cobalt, strontium, barium, arsenic and
selenium were determined. The results obtained by LIBS were compared with the
ICP-MS results both directly and using machine learning algorithms. According
to the results, LIBS spectra did not correlate with heavy metal levels obtained
by ICP-MS. It was considered that the heavy metal levels in the blood samples
were below the limits of detection of the LIBS method applied. As an additional
finding in the study, it has been shown that LIBS spectra of different tissues
can be differentiated by machine learning. It was concluded that; in order to
use the LIBS method effectively in determining the levels of heavy metals in
tissues, studies should be carried out to improve the limits of detection,
separately for each element.