The ability to separate isomer series and the surface properties of cholesteryl chloroformate (CC) liquid crystal were investigated by inverse gas chromatography (IGC). The retention diagrams of the selected solvents were plotted between 308.2 and 333.2 K using the IGC technique. The selectivity coefficients, alpha, of the structural isomers were determined for the CC liquid crystal in the infinite dilution. Besides, the dispersive energy, gamma(D)(S), the Gibbs specific energy, Delta G(A)(S), the adsorption enthalpy, Delta H-A(S), the adsorption entropy, Delta S-A(S), of the selected solvents were also determined. The Delta H-A(S) values were associated with the number of acidic and basic group to calculate the acidity constant (K-A) and the basicity constant (K-D). When the Delta H-A(S) values calculated in experimental studies were examined, it was seen that the adsorption takes place exothermically. According to K-A and K-D constants, CC surface was found to be basic character (K-D/K-A similar or equal to 3.11).