Synthesis and characterization of cellobiose-derived oligosaccharides with Bifidogenic activity by glucansucrase E81
FOOD BIOSCIENCE, cilt.44, 2021 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 44
- Basım Tarihi: 2021
- Doi Numarası: 10.1016/j.fbio.2021.101388
- Dergi Adı: FOOD BIOSCIENCE
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Food Science & Technology Abstracts, INSPEC
- Anahtar Kelimeler: Glucansucrase, Prebiotic oligosaccharides, Cellobiose-derived oligosaccharides, STRUCTURAL-CHARACTERIZATION, GALACTO-OLIGOSACCHARIDES, ACCEPTOR REACTIONS, ALTERNANSUCRASE, LACTOSE, SUCROSE, GROWTH, DEXTRANSUCRASE, TRISACCHARIDE, FERMENTATION
- Yıldız Teknik Üniversitesi Adresli: Evet
Özet
Cellobiose-derived oligosaccharides were produced from acceptor reactions with glucansucrase E81 and their prebiotic potentials were determined in this study. The major product was a mixture of trisaccharides formed by the addition of an alpha-linked glucose to cellobiose at either the 2-O position of the reducing end (86%) glucose or the 6-O position of the non-reducing end glucose (11%) demonstrated by NMR spectroscopy and LC-MS-LTQ analysis. The purified DP 3 cellobiose-derived oligosaccharides were then tested for in vitro human gastric juice and alpha-amylase digestion and no digestion was observed during the 6 h digestion period. Importantly, DP 3 cellobiose-derived oligosaccharides promoted the growth of Bifidobacteria at high levels demonstrating a Bifidogenic effect and a mild level of proliferation was observed for Lactobacilli suggesting the prebiotic roles of cellobiose-derived oligosaccharides.