Model-based fractionation of biomass in a biological nutrient removal system and its effect on the removal efficiencies

Manav Demir N.

Journal of Environmental Health Science and Engineering, vol.21, no.1, pp.123-132, 2023 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 21 Issue: 1
  • Publication Date: 2023
  • Doi Number: 10.1007/s40201-022-00845-8
  • Journal Name: Journal of Environmental Health Science and Engineering
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.123-132
  • Keywords: Autotrophs, Heterotrophs, Phosphorus accumulating organisms, Principal component analysis, Wastewater treatment
  • Yıldız Technical University Affiliated: Yes


Fractionation of active biomass in a five-stage Bardenpho process was accomplished using an MS Excel wastewater treatment plant modeling tool based on Activated Sludge Model No. 3 extended with a bio-P module. The biomass fractions within the treatment system were predicted as autotrophs, ordinary heterotrophs, and phosphorus accumulating organisms (PAOs). Several simulations were performed in a Bardenpho process using various C/N/P ratios in primary effluent. Biomass fractionation was obtained from steady-state simulation results. The results suggest that the mass percentage of autotrophs, heterotrophs, and PAOs in active biomass range from 1.7 to 7.8%, 5.7–69.0%, and 23.2–92.6%, respectively, depending on characteristics of primary effluent. Results of principal component analysis showed that TKN/COD ratio in primary effluent determines the population of autotrophs and ordinary heterotrophs whereas PAO population is mainly a function of TP/COD ratio.