Fabrication of hemp seed oil-doped gelatin/sodium alginate nanofibers using the Box-Behnken experimental design

Usta M., Erol Taygun M. M., Yılmaz E.

5th International Eurasian Conference on Biological and Chemical Sciences, Ankara, Turkey, 23 - 25 November 2022, pp.344

  • Publication Type: Conference Paper / Summary Text
  • City: Ankara
  • Country: Turkey
  • Page Numbers: pp.344
  • Yıldız Technical University Affiliated: Yes


Hemp seed oil is a nutritious oil containing over 80% polyunsaturated fatty acids obtained by cold pressing from the seeds

of the Cannabis Sativa L. plant. It contains omega-6 fatty acids such as linoleic acid (LA, 54%), gamma-linolenic acid

(GLA, 3%) and omega-3 fatty acids (alpha-linoleic acid (ALA, 17%) and stearidonic acid (2%). The hemp seed oil shows

pharmacological activity due to the benefits it provides. The antibacterial property of the oil is proven against gramnegative

bacteria Escherichia coli (E.Coli) and gram-positive bacteria Staphylococcus auerus (S.Aureus) according to

the disc diffusion method. Due to this antibacterial feature, hemp seed oil is expected to provide an antibacterial wound

healing environment and accelerate healing thanks to the components it contains. Therefore, in the present study, it was

aimed to investigate the use of hemp seed oil in a wound dressing system. For this purpose, it was tried to obtain a wound

dressing system with nanofiber structure with the most suitable fiber diameter by encapsulating hemp seed oil into

gelatin/sodium alginate nanofibers by electrospinning method. The antibacterial activity test results showed that the

antibacterial activity decreased with the decrease in the oil concentration. Furthermore, three-level, three-variable Box-

Behnken design-based response surface methodology was employed as an optimization tool to select the most appropriate

parameter settings required to obtain the desired fiber diameter. Accordingly, the diameter of the nanofibers obtained

from the solutions prepared at different component concentrations based on the experimental design was investigated by

using scanning electron microscopy (SEM) analysis and a second-order polynomial equation was developed to estimate

the fiber diameter. It was found that the experimental data and the developed models were in good agreement. The

effectiveness of the nanofiber wound dressing material fabricated under optimum conditions based on the design results

will be supported by future characterization tests.