Akademik Veri Yönetim Sistemi
International Conference on New Trends in Science and Applications (NTSA), New York, Amerika Birleşik Devletleri, 12 - 13 Ekim 2021, ss.31
People's lifestyle, the environment they live in and
the products they use cause changes in their gene expression. Changes in living
conditions have a direct impact on the body. One of the most affected processes
is the aging process. In our research we examined the effect of curcumin in the
aging process of human skin fibroblasts. Aging is directly related to many
changes in the cell. DNA is exposed to changes such as shortening of telomeres,
chromatin changes, DNA methylation, and some of these changes can be used as
biomarkers. Within the scope of this research, the effect of curcumin (which is
known to be anti-aging in dermal fibroblasts) in the expression level of target
gene is analyzed. Two groups were used; the control group not treated with any
agent and the experimental group exposed to the anti-aging agent (curcumin).
Normal ageing process will be used, followed by total RNA isolation from the
cells incubated under appropriate conditions and then the expression level of
the target gene, HAS2 analyze is done by using real-time polymerase chain
reaction.