© 2022 Kaunas University of Technology. All rights reserved.Istanbul is one of the most crowded cities in the world, located at the crossroads of two continents, Europe and Asia. The city has high passenger mobility, however getting from one side of the city to the other is difficult, especially during working hours. Maritime transportation, which is done from various ports, is one of the modes of transportation used for passenger transit. To conduct the analysis, a liner passenger transit route in İstanbul Strait was chosen. Transportation operations in the Strait have an impact on both the environment and human health that living around. The International Maritime Organization, in particular, has established limits on CO2, NOx, SOx, and PM emissions within the scope of the Paris Agreement Objectives, which are emitted by ships. In addition to the Initial IMO Strategy on the reduction of GHG emissions from ships, the European Union has also published a series of measures via the European Green Deal, including waterborne transport, to make Europe the world's first climate-neutral continent by 2050. Electrification, or the use of electricity as a power source, is an efficient technique for lowering shipping emissions and allowing cleaner transportation. In this sense, maritime travel between two nearby terminals has been investigated in this study since short-sea transportation is well-suited to electrification. Furthermore, a reference energy system for the vessel operating on this route has been built, and it has been analysed and simulated in terms of technical, economic, and environmental aspects using the Low Emissions Analysis Platform (LEAP). On the other side, an alternative scenario has been designed and implemented to demonstrate the potential consequences of electricity in the Istanbul Strait.