A 2,7-dichlorofluorescein derivative to monitor microcalcifications

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Tholen P., Brown C. N., Keil C., Bayir A., Zeng H., Haase H., ...Daha Fazla

MOLECULAR SYSTEMS DESIGN & ENGINEERING, cilt.7, sa.11, ss.1415-1421, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 7 Sayı: 11
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1039/d2me00185c
  • Dergi Adı: MOLECULAR SYSTEMS DESIGN & ENGINEERING
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Compendex, INSPEC
  • Sayfa Sayıları: ss.1415-1421
  • Yıldız Teknik Üniversitesi Adresli: Evet

Özet

Herein, we report the crystal structure of 2,7-dichlorofluorescein methyl ester (DCF-ME) and its fluorescence response to hydroxyapatite binding. The reported fluorophore is very selective for staining the bone matrix and provides turn-on fluorescence upon hydroxyapatite binding. The reported fluorophore can readily pass the cell membrane of the C2C12 cell line, and it is non-toxic for the cell line. The reported fluorophore DCF-ME may find applications in monitoring bone remodeling and microcalcification as an early diagnosis tool for breast cancer and age-related macular degeneration.