A 2,7-dichlorofluorescein derivative to monitor microcalcifications
MOLECULAR SYSTEMS DESIGN & ENGINEERING, vol.7, no.11, pp.1415-1421, 2022 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 7 Issue: 11
- Publication Date: 2022
- Doi Number: 10.1039/d2me00185c
- Journal Name: MOLECULAR SYSTEMS DESIGN & ENGINEERING
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Compendex, INSPEC
- Page Numbers: pp.1415-1421
- Open Archive Collection: AVESIS Open Access Collection
- Yıldız Technical University Affiliated: Yes
Abstract
Herein, we report the crystal structure of 2,7-dichlorofluorescein methyl ester (DCF-ME) and its fluorescence response to hydroxyapatite binding. The reported fluorophore is very selective for staining the bone matrix and provides turn-on fluorescence upon hydroxyapatite binding. The reported fluorophore can readily pass the cell membrane of the C2C12 cell line, and it is non-toxic for the cell line. The reported fluorophore DCF-ME may find applications in monitoring bone remodeling and microcalcification as an early diagnosis tool for breast cancer and age-related macular degeneration.