Polymer inclusion membranes are a type of liquid membrane that is used to separate and extract cations and anions. For instance, these are used to separate toxic metal wastes from the environment. 1,10-Phenanthroline has been used as a preferred classic chelated ligand for transition metal ions. In this study, we prepared a cellulose tri-acetate (CTA) polymeric membrane containing 1,10-phenanthroline. We used a combination of CTA membrane with polyethylene glycol to extract metals from aqueous solutions using a two-compartment permeation cell having a feed and stripping solution. We used cobalt, nickel, copper, and cadmium metals to prepare the feed solution, whereas ammonia and tri-ethanolamine were used to prepare the stripping solution. Cadmium remains in the feed solution, whereas cobalt, nickel, and copper are transferred to the stripping phase. The extraction efficiencies of metals were found to be in the order Co > Ni > Cu. This method resulted in the extraction of 91% cobalt, 89% nickel, and 89% copper in an aqueous solution. In addition, the modified membranes were characterized for their compositional analysis and surface morphology. Fourier transform infrared spectroscopy was used to determine the presence of functional groups.