Analyses of abiotic stress and brassinosteroid-related some genes in barley roots grown under salinity stress and HBR treatments: Expression profiles and phylogeny

Marakli S., Gozukirmizi N.

PLANT BIOSYSTEMS, cilt.152, sa.3, ss.324-332, 2018 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 152 Sayı: 3
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1080/11263504.2016.1271054
  • Dergi Adı: PLANT BIOSYSTEMS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.324-332
  • Anahtar Kelimeler: Aquaporins, CYCD3, DREB2, DWARF4, Hordeum vulgare L, WAK, KINASE GENE, PLANT AQUAPORINS, WATER TRANSPORT, PROTEIN-KINASE, ARABIDOPSIS, DROUGHT, HOMEOSTASIS, GERMINATION, MECHANISMS, PLAYS
  • Yıldız Teknik Üniversitesi Adresli: Hayır

Özet

We aimed to investigate abiotic stress (WAK, HvPIP1.1, HvPIP1.2, HvPIP1.3, HvPIP1.5, CYCD3, DREB2) and brassinosteroid-related gene (DWARF4) expressions in barley (Hordeum vulgare L. cv. Hilal) roots grown under different salt (150 and 250mM), HBR (0.5 and 1M), and salt+HBR applications during 48 and 72h at dark with their controls. Phylogenetic trees were also constructed to observe relationships among genes found in other plants. The expression of HvPIP1.2 and WAK reduced after salt treatment while HvPIP1.3, DREB2 and DWARF4 expressions increased. HvPIP1.1, HvPIP1.2, HvPIP1.3, HvPIP1.5 and DWARF4 expressions were upregulated under only HBR applications. Salt+HBR treatments increased HvPIP1.1, DREB2 and DWARF4 but decreased HvPIP1.2. Phylogenetic analyses indicated that Oryza sativa L. shared similar sequences with HvPIP1.5. CYCD3 could diverge relatively earlier from cyclin genes during evolution as it segregates in a distinct clade. Sorghum bicolor showed sequence homology with DREB2. Oryza australiensisL. and DWARF4 were found in the same clade. To our knowledge, this is the first detailed report related to salt stress and HBR applications in terms of the expression of different genes in barley, providing a valuable information for molecular breeding improvement of stress-related traits.