Simultaneous determination of mepyramine maleate, lidocaine hydrochloride, and dexpanthenol in pharmaceutical preparations by partial least-squares multivariate calibration

Aksu O., Bozdogan A. E., KUNT G.

ANALYTICAL LETTERS, vol.39, no.4, pp.751-761, 2006 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 39 Issue: 4
  • Publication Date: 2006
  • Doi Number: 10.1080/00032710600611210
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.751-761
  • Yıldız Technical University Affiliated: Yes


A partial least-squares calibration ( PLS) method has been developed for simultaneous quantitative determination of mepyramine maleate ( MAM), lidocaine hydrochloride ( LIH), and dexpanthenol ( DPA) in pharmaceutical preparations. The resolution of these mixtures has been accomplished by using partial least-squares ( PLS-2) regression analysis of electronic absorption spectral data without prior separation or derivatization. The experimental calibration matrix was constructed with 27 samples. The concentration ranges considered were 2, 3, 4 mu gmL(-1) for MAM, 2, 3, 4 mu gmL(-1) for LIH, and 8, 10, 12 mu gmL(-1) for DPA. The absorbances were recorded between 190 and 340 nm every 5 nm. The results show that PLS-2 is a simple, rapid, and accurate method applied to the determination of these compounds in pharmaceuticals.