A novel fluorometric measurement system based on triple complex for mercury (II) determination

Toksöz Y. S., ÖZYİĞİT İ. E., BİLEN Ç., ARSU N., KARAKUŞ E.

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, vol.248, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 248
  • Publication Date: 2021
  • Doi Number: 10.1016/j.saa.2020.119232
  • Journal Name: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Compendex, EMBASE, INSPEC, MEDLINE, Veterinary Science Database
  • Keywords: 2-Thioxanthone thioacetic acid, Bovine serum albumin, Fluorescence spectroscopy, Mercury (II), Trace analysis, ACETIC-ACID DERIVATIVES, FLUORESCENCE SPECTROSCOPY, SERUM-ALBUMIN, THIOXANTHONE, POLYMERIZATION
  • Yıldız Technical University Affiliated: Yes

Abstract

T-BSA binary complex was formed with non-covalent interactions between fluorometric 2-thioxanthone thioacetic acid and stabilizator bovine serum albumin by fluorescence emission spectroscopy as a sensible and practical method. T-BSA concentration at 1.77 x 10(-4) M was obtained as the most suitable and reliable amount for the formation of T-BSA-Hg(II) triple complex. Trace amount of Hg(II) analyses were achieved by this new fluorometric triple complex system as the primary aim. The emission spectra from 350 nm to 650 nm were assayed on fluorometer for Hg(II) concentrations from 1.77 x 10(-8) M to 3.53 x 10(-4) M under an excitation wavelength of 337 nm. Hg(II) was found to increase the emission intensity of T-BSA by 50% even at 1.77 x 10(-7) M Hg(II). So this new system has strong sensitivity to Hg(II) ion. T-BSA-Hg(II) triple complex formation and its fluorometric characterization have not been investigated in literature yet. This study is critically important to provide Hg(II) analyses in wastewater treatments and biological samples for further studies. (C) 2020 Elsevier B.V. All rights reserved.