A novel fluorometric measurement system based on triple complex for mercury (II) determination


Toksöz Y. S. , ÖZYİĞİT İ. E. , BİLEN Ç. , ARSU N. , KARAKUŞ E.

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, vol.248, 2021 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 248
  • Publication Date: 2021
  • Doi Number: 10.1016/j.saa.2020.119232
  • Title of Journal : SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

Abstract

T-BSA binary complex was formed with non-covalent interactions between fluorometric 2-thioxanthone thioacetic acid and stabilizator bovine serum albumin by fluorescence emission spectroscopy as a sensible and practical method. T-BSA concentration at 1.77 x 10(-4) M was obtained as the most suitable and reliable amount for the formation of T-BSA-Hg(II) triple complex. Trace amount of Hg(II) analyses were achieved by this new fluorometric triple complex system as the primary aim. The emission spectra from 350 nm to 650 nm were assayed on fluorometer for Hg(II) concentrations from 1.77 x 10(-8) M to 3.53 x 10(-4) M under an excitation wavelength of 337 nm. Hg(II) was found to increase the emission intensity of T-BSA by 50% even at 1.77 x 10(-7) M Hg(II). So this new system has strong sensitivity to Hg(II) ion. T-BSA-Hg(II) triple complex formation and its fluorometric characterization have not been investigated in literature yet. This study is critically important to provide Hg(II) analyses in wastewater treatments and biological samples for further studies. (C) 2020 Elsevier B.V. All rights reserved.