Histopathological, immunohistochemical, and biomechanical effects of splenectomy on Achilles tendon healing in rats

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Yüksel S., Gulec M. A. , Gültekin Z., Çağlar A., Beytemur O., Alagöz E., ...More

CONNECTIVE TISSUE RESEARCH, vol.60, pp.200-208, 2019 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 60
  • Publication Date: 2019
  • Doi Number: 10.1080/03008207.2018.1483361
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.200-208
  • Keywords: Achilles tendon, cytokines, growth factor, rats, splenectomy, LYMPHOCYTE FUNCTION, IFN-GAMMA, TNF-ALPHA, BONE LOSS, CELLS, EXPRESSION, IMMUNE, RUPTURES, SPLEEN, REPAIR


Purpose: This study aimed to assess Achilles tendon repair in rats following splenectomy to simulate patients with musculoskeletal system injury who had splenectomy after spleen injury, a situation often seen in orthopedics and traumatology practice. Materials and Methods: The study included 32 male Sprague-Dawley rats (10 months old; average weight, 394.5 +/- 28.3 g). The rats were fed with standard rodent food ad libitum at 22 degrees C in a dark environment for 12 h. They were divided into two groups, namely the splenectomy (total splenectomy and Achilles tendon repair) and control groups (only Achilles tendon repair; n = 16). Four weeks after the surgery, the rats were euthanized, and their Achilles tendons were examined histopathologically, immunohistochemically, and biomechanically. Results: In the splenectomy group, proinflammatory cytokines, such as interleukin-1 beta, tumor necrosis factor-alpha, and interferon-gamma, showed significantly lower values than those in the control group (p <0.01); moreover, the levels of anti-inflammatory cytokines like vascular endothelial growth factor, transforming growth factor-beta 1, interleukin-2, interleukin-10, and hepatocyte growth factor were significantly higher than in the control group (p < 0.001). The average ultimate tensile strengths were 2.58 +/- 0.5 in the splenectomy and 2.78 +/- 0.3 in the control group (p = 0.043). The average values were 0.33 +/- 0.5 in the splenectomy and 0.44 +/- 0.1 in the control group (p = 0.021). Conclusion: Splenectomy may positively influence Achilles tendon healing through modification of the proinflammatory/anti-inflammatory ratio in favor of anti-inflammatory cytokines by causing a decrease in spleen-originated inflammatory cells.