Development of Fe3O4/reduced graphene oxide nanocomposite-based dispersive solid-phase extraction for the quantification of five steroid hormones

Çağatay N. U., Maviş M. E., ERARPAT S., BAKIRDERE S.

Bioanalysis, vol.15, no.9, pp.503-512, 2023 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 15 Issue: 9
  • Publication Date: 2023
  • Doi Number: 10.4155/bio-2023-0025
  • Journal Name: Bioanalysis
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core, EMBASE, MEDLINE
  • Page Numbers: pp.503-512
  • Keywords: dispersive solid-phase extraction, liquid chromatography–tandem mass spectrometry, magnetic nanoparticles, serum samples, steroid hormones
  • Yıldız Technical University Affiliated: Yes

Abstract

Aim: An accurate and sensitive analytical method was proposed to detect some steroid hormones in biological samples. Materials & methods: An Fe3O4/reduced graphene oxide nanocomposite-based dispersive solid-phase extraction was developed for the effective and simple preconcentration of steroid hormones from human serum samples. Results & conclusion: The nanocomposite was firstly used as adsorbent to simultaneously extract the selected hormones. Limit of detection values for the selected hormones were calculated between 5.5 and 39.2 ng/kg (mass based). An artificial serum sample was used to test the applicability and accuracy of the developed method; percentage recovery results obtained from two different spiked concentrations were found to be in the range of 80.5-99.9%.