Development of Fe3O4/reduced graphene oxide nanocomposite-based dispersive solid-phase extraction for the quantification of five steroid hormones
Bioanalysis, vol.15, no.9, pp.503-512, 2023 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 15 Issue: 9
- Publication Date: 2023
- Doi Number: 10.4155/bio-2023-0025
- Journal Name: Bioanalysis
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core, EMBASE, MEDLINE
- Page Numbers: pp.503-512
- Keywords: dispersive solid-phase extraction, liquid chromatography–tandem mass spectrometry, magnetic nanoparticles, serum samples, steroid hormones
- Yıldız Technical University Affiliated: Yes
Abstract
Aim: An accurate and sensitive analytical method was proposed to detect some steroid hormones in biological samples. Materials & methods: An Fe3O4/reduced graphene oxide nanocomposite-based dispersive solid-phase extraction was developed for the effective and simple preconcentration of steroid hormones from human serum samples. Results & conclusion: The nanocomposite was firstly used as adsorbent to simultaneously extract the selected hormones. Limit of detection values for the selected hormones were calculated between 5.5 and 39.2 ng/kg (mass based). An artificial serum sample was used to test the applicability and accuracy of the developed method; percentage recovery results obtained from two different spiked concentrations were found to be in the range of 80.5-99.9%.