Comparison of extraction methods optimised by RSM for extraction of some bioactives from liquorice samples

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Ozcan F. S., Ozcan N., Us A. A., SAĞDIÇ O.

TURKISH JOURNAL OF AGRICULTURE AND FORESTRY, vol.44, no.1, pp.24-38, 2020 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 44 Issue: 1
  • Publication Date: 2020
  • Doi Number: 10.3906/tar-1903-46
  • Journal Name: TURKISH JOURNAL OF AGRICULTURE AND FORESTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Compendex, Environment Index, Geobase, INSPEC, Veterinary Science Database, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.24-38
  • Keywords: LC-ESI-MS/MS, liquorice, microwave, response surface methodology ultrasound, MICROWAVE-ASSISTED EXTRACTION, GLYCYRRHIZIC ACID, NATURAL SWEETENER, ROOT, QUANTIFICATION, CONSTITUENTS, INGREDIENT, DESIGN, GREEN
  • Yıldız Technical University Affiliated: Yes

Abstract

This study compared the extraction efficiency of 3 different techniques, ultrasound-assisted extraction (UAE), microwave-assisted extraction (MAE), and the classical extraction method, in obtaining some bioactive and allergen compounds, namely liquiritin (LQ), glycyrrhizin (GL), glycyrrhetinic acid (GA), and carbenoxolone (CBX), from liquorice samples of Glycyrrhiza glabra grown in Turkey. Extraction parameters for each technique were optimised by the response surface methodology-central composite design approach. Concentrations of LQ, GL, GA, and CBX, which were selected as the responses of selected independent variables, were determined by liquid chromatography-electrospray ionisation tandem mass spectrometry. One-way variance analysis was performed to evaluate the significance of the results. MAE was revealed as the most effective technique for extracting LQ (mean: 8.52 mg g(-1)), GL (mean: 76.65 mg g(-1)), and GA (mean: 1138.84 mu g g(-1)) from liquorice, while the classical technique was the most effective for obtaining CBX (mean: 5.75 mu g g(-1)).