Development of humic acid and alginate-based wound dressing and evaluation on inflammation


Yalman V., Türkoğlu Laçin N.

MATERIALS TECHNOLOGY, cilt.34, sa.12, ss.705-717, 2019 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 34 Sayı: 12
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1080/10667857.2019.1619961
  • Dergi Adı: MATERIALS TECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.705-717
  • Yıldız Teknik Üniversitesi Adresli: Evet

Özet

The excessive collagen type I accumulation in the extracellular matrix leads to scar formation. We aimed to develop a biomaterial for scarless tissue regeneration by suppressing collagen type I overexpression, and stimulation of collagen type III expression. Alginate (Alg) is a widely used natural material for biomedical applications, and humic acid (HA) is a natural macromolecule with antioxidant, anti-inflammatory properties. Humic acid and the alginate were cross-linked to obtain a hydrogel membrane. The cell viability of the Humic Acid-Alginate (HA-Alg) hydrogel on L929, HaCaT, macrophage cells were determined. The biodegradation tests revealed that 76% of the HA-Alg hydrogel mass was lost at four weeks The effect of HA-Alg hydrogel on TNF alpha level observed as 1212.50 +/- 98.63 pg/mL. HA-Alg suppressed collagen type I overexpression, which has a significant role in scar formation and stimulated collagen type III expression, which has a vital role in wound healing.