Development of humic acid and alginate-based wound dressing and evaluation on inflammation

Yalman V., Türkoğlu Laçin N.

MATERIALS TECHNOLOGY, vol.34, no.12, pp.705-717, 2019 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 34 Issue: 12
  • Publication Date: 2019
  • Doi Number: 10.1080/10667857.2019.1619961
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.705-717
  • Yıldız Technical University Affiliated: Yes


The excessive collagen type I accumulation in the extracellular matrix leads to scar formation. We aimed to develop a biomaterial for scarless tissue regeneration by suppressing collagen type I overexpression, and stimulation of collagen type III expression. Alginate (Alg) is a widely used natural material for biomedical applications, and humic acid (HA) is a natural macromolecule with antioxidant, anti-inflammatory properties. Humic acid and the alginate were cross-linked to obtain a hydrogel membrane. The cell viability of the Humic Acid-Alginate (HA-Alg) hydrogel on L929, HaCaT, macrophage cells were determined. The biodegradation tests revealed that 76% of the HA-Alg hydrogel mass was lost at four weeks The effect of HA-Alg hydrogel on TNF alpha level observed as 1212.50 +/- 98.63 pg/mL. HA-Alg suppressed collagen type I overexpression, which has a significant role in scar formation and stimulated collagen type III expression, which has a vital role in wound healing.