Particle size and zeta potential of polyelectrolyte-protein binary conjugate and polyelectrolyte-metal-protein ternary complexes
are among the most important factors affecting biopharmaceutical behavior such as bioavailability, dissolution and immune
toxicity of these complexes. For this purpose, in the current study, the interactions between binary and ternary anionic polyelectrolyte, “polyacrylic acid (PAA)”, BSA and copper ions in different salt concentrations were investigated by zetasizer analysis. The particle size of poly(acrylic acid)-copper (II)-bovine serum albumin (BSA) ternary complex in a salt solution was modeled
using extensive regression methods from Partial Least Squares (PLS) regression to conventional multiple linear regression.