In this study, we have focused on the use of a poly(L-cysteine) (PLC) modified pencil graphite electrode (PGE) as a sensor for determination of Sunset Yellow (SY). The performance of the modified electrode was studied using cyclic voltammetric and differential pulse voltammetric method. The modified electrode was characterized by electrochemical impedance spectroscopy, cyclic voltammetry and Fourier transform infrared spectroscopy. The surface of the modified electrode was examined by scanning electron microscope. The electrochemical behavior of SY in phosphate buffer solution (pH 7.0) was examined using unmodified PGE and PLC modified PGE (PLC/PGE). The results showed that the electrochemical response of PLC/PGE to SY was significantly developed. The PLC/PGE showed linear responses in the electrochemical oxidation of SY between the concentration values of 1.0 mu M and 1000 mu M The sensor (PLC/PGE) showed a good response for SY with a detection limit of 0.125 mu M (S/N=3). Analytical application of PLC/PGE was successfully tested in the determination of SY in food and beverage samples.