Antioxidant Activity and Hemocompatibility Study of Quercetin Loaded Plga Nanoparticles

DERMAN S., Uzunoglu D., Acar T., ARASOĞLU T., Ucak S., Ozalp V. C., ...Daha Fazla

IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH, cilt.19, sa.1, ss.424-435, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 19 Sayı: 1
  • Basım Tarihi: 2020
  • Doi Numarası: 10.22037/ijpr.2020.1101000
  • Dergi Adı: IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, EMBASE, International Pharmaceutical Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.424-435
  • Anahtar Kelimeler: Quercetin, Polymeric nanoparticle, Hemocompatibility, Antioxidant activity, Hemolytic activity, PARTICLE-SIZE, BIODEGRADABLE NANOPARTICLES, POLYMERIC NANOPARTICLES, FLAVONOID ANTIOXIDANT, DRUG-DELIVERY, FORMULATION, BIOAVAILABILITY, HYDROCHLORIDE, OPTIMIZATION, PARAMETERS
  • Yıldız Teknik Üniversitesi Adresli: Evet

Özet

Quercetin (QU) is an important flavonoid compound presenting lots of biological activities, but its application has been limited due to its low aqueous solubility and instability. In this study, conducted to improve these properties of the quercetin, quercetin-encapsulated PLGA nanoparticles were prepared, characterized, and evaluated for antioxidant and hemolytic activity. Nanoparticles were produced by single emulsion solvent evaporation method. Four different process parameters initial QU amount, PVA concentration, PVA volume, and initial PLGA amount were investigated to obtain the nanoparticles which have minimum particle size and maximum entrapment efficiency. Synthesized nanoparticles were evaluated for particle size, entrapment efficiency, and reaction yield. Additionally, antioxidant properties and in-vitro hemolytic activity of quercetin loaded nanoparticles with different particle size were also evaluated for the first time in the literature. The antioxidant activity results showed that nanoparticles have different antioxidant activity, depending on the amount of quercetin release from nanoparticles at different particle sizes. The hemolytic activity results show that all nanoparticles exhibited favorable compatibility to red blood cells and no significant hemolytic effect was observed.