Development of a double monitoring system for the determination of Cr(VI) in different water matrices by HPLC-UV and digital image-based colorimetric detection method with the help of a metal sieve-linked double syringe system in complexation


UNUTKAN GÖSTERİŞLİ T., Oflu S., KEYF S., BAKIRDERE S.

ENVIRONMENTAL MONITORING AND ASSESSMENT, vol.194, no.10, 2022 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 194 Issue: 10
  • Publication Date: 2022
  • Doi Number: 10.1007/s10661-022-10392-2
  • Journal Name: ENVIRONMENTAL MONITORING AND ASSESSMENT
  • Journal Indexes: Science Citation Index Expanded, Scopus, ABI/INFORM, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Compendex, EMBASE, Environment Index, Food Science & Technology Abstracts, Geobase, Greenfile, MEDLINE, Pollution Abstracts, Public Affairs Index, Veterinary Science Database, Civil Engineering Abstracts
  • Keywords: Chromium, High-performance liquid chromatography, Digital image-based colorimetry, Metal sieve-linked double syringe, Water samples, LIQUID-LIQUID-EXTRACTION, CLOUD POINT EXTRACTION, SPECIATION, CHROMIUM, CR(III), PRECONCENTRATION, TOXICITY

Abstract

This study reports a cheap, efficient, sensitive, and simple double monitoring analytical method for trace determination of Cr(VI), which is toxic and harmful even at very low concentrations. A metal sieve-linked double syringe (MSLDS) system was used to help the formation of chromium complex (Cr-diphenyl carbazide, DPC) subsequently determined by high-performance liquid chromatography-ultraviolet (HPLC-UV) and digital image-based colorimetry (DIC) systems. The metal complex was eluted through a Phenomenex-Aqua C18 with a mobile phase comprising of 50 mM ammonium formate solution (pH 4.0):acetonitrile (78:22, v/v) and detected by the UV detector at the wavelength of 581 nm. Under their optimum conditions, the HPLC-UV and DIC systems exhibited good linearity in ranges of 10-500 mu g L-1 and 100-1000 mu g L-1, respectively. The percent relative standard deviations (RSD%s) calculated for the lowest concentrations of both systems fell below 10%, and this confirmed good repeatability for replicate measurements. The accuracy of the proposed methods was evaluated by performing spike recovery experiments on wastewater, river water, and tap water samples. The calculated recovery results were in the range of 81.5-105.5% for HPLC-UV system and 93.8-111.1% for the DIC system. These results indicate that the proposed methods are suitable for routine Cr(VI) determination in terms of their rapidness, simplicity, good repeatability, and low cost.