Molecular-weight distribution and structural transformation in water-soluble complexes of poly(acrylic acid) and bovine serum albumin
EUROPEAN POLYMER JOURNAL, cilt.43, sa.7, ss.2935-2946, 2007 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 43 Sayı: 7
- Basım Tarihi: 2007
- Doi Numarası: 10.1016/j.eurpolymj.2007.04.025
- Dergi Adı: EUROPEAN POLYMER JOURNAL
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.2935-2946
- Anahtar Kelimeler: polyelectrolyte, protein, polycomplex, size-exclusion chromatography with quadruple detection, SIZE-EXCLUSION CHROMATOGRAPHY, PERFORMANCE LIQUID-CHROMATOGRAPHY, LIGHT-SCATTERING, ANTIGEN-SPECIFICITY, POLYACRYLIC-ACID, HYBRIDOMA CLONES, IMMUNE-RESPONSE, PROTEIN COMPLEX, SYNTHETIC POLYELECTROLYTES, AFFINITY
- Yıldız Teknik Üniversitesi Adresli: Evet
Özet
Interaction of polyacrylic acid (PAA) with bovine serum albumin (BSA) at different pH values and in a wide range of mixing molar ratios, gamma = n(BSA)/n(PAA), of components was investigated by size-exclusion high performance liquid chromatography with on-line refractive index, UV, light scattering and viscometer detectors. The results revealed the formation of stable water-soluble polymer-protein complexes at pH 5.0. For the soluble complexes thus formed, the number of the bound BSA molecules with one PAA molecule was expressed by a Langmuir-type equation as a function of the amount of excess BSA existing free in the solution. At saturation, one BSA molecule is bound to about 48 acrylic acid residues.