The aim of this study was to investigate the in vivo dose-dependent effects of Matricaria chamomilla extract (MCE) on cellular trace and major element concentrations lit ethanol-intoxicated rats. Firstly, MCE (0, 25, 50, 100, 200 or 400 mg/kg) or famotidine (20 mg/kg) was administered to male rats (n = 8, in each group) by gavage (0.5 mL) one hour before 80% ethanol gavage (1 mL) then the erythrocyte concentrations of major elements (K(+), Ca(2+) and Mg(2+)) and of trace elements (Cu, Zn, Al, Mn, Cr, Se and Ni) were measured by optical emission spectroscopy one hour later and compared to healthy controls (n = 8). The K, Cu, Zn and Al cellular contents did not significantly varied among the different groups. Compared to the healthy controls (untreated and not intoxicated), the famotidine pre-treatment induced significant reductions of the cellular Ca and Mg concentrations and significant increase of the Se content whereas the MCE administration did not significantly after these biochemical parameters. The Cr and Mn contents tended to increase in rats only intoxicated with ethanol and in rats treated with the lowest and the highest doses of MCE whereas famotidine tended to depress them. Finally, compared to ethanol intoxicated animals, famotidine and MCE (at doses of 100, 200 and 400 mg/kg) pre-treatments have restored the erythrocyte Ni contents which became similar to control values. These results suggest that the MCE treatment. mainly at high doses, may prevent alterations of the mineral status induced by ethanol in rats with the same or even it greater efficiency than the drug reference (famotidine).