A comparison of metabolic engineering strategies applied in Yarrowia lipolytica for β-carotene production

Uçar R. A., Demirgül F., ŞİMŞEK Ö., ERTEN H.

Biotechnology and Bioprocess Engineering, vol.29, no.1, pp.69-83, 2024 (SCI-Expanded) identifier

  • Publication Type: Article / Review
  • Volume: 29 Issue: 1
  • Publication Date: 2024
  • Doi Number: 10.1007/s12257-024-00006-8
  • Journal Name: Biotechnology and Bioprocess Engineering
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, ABI/INFORM, Applied Science & Technology Source, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Compendex, EMBASE, Food Science & Technology Abstracts, Veterinary Science Database
  • Page Numbers: pp.69-83
  • Keywords: Cell factory, Engineering strategies, Yarrowia lipolytica, β-Carotene
  • Yıldız Technical University Affiliated: Yes


With a better understanding of the health benefits of β-carotene, the precursor of vitamin A, as well as its coloring property, the need for this carotenoid has increased in various sectors. In order to meet the increasing demand efficiently, cheaply, and sustainably, interest in heterologous β-carotene production through metabolic engineering strategies has increased in recent years. In this context, although it is not a native producer of β-carotene, Yarrowia lipolytica yeast stands out due to its metabolic, physiological, and genomic properties. Successful results have been obtained by using a series of engineering strategies, including biosynthesis pathway engineering, morphological engineering, and fermentation engineering strategies, in the production of heterologous β-carotene from Y. lipolytica. However, these strategies have various strengths and weaknesses against each other, and there are also some points open to improvement. In this review, the engineering strategies that have been applied and have the potential to be applied for the production of β-carotene from Y. lipolytica have been examined in depth, including their advantages and disadvantages, and compared with each other. Moreover, a future perspective has been presented to increase the potential of using Y. lipolytica yeast as a cell factory in β-carotene production.