Immuno-histochemical, pathological, enzyme linked immunosorbent assay and polymerase chain reaction analysis of experimental Ornithobacterium rhinotracheale infection in quails (Coturnix coturnix japonica)


Eroksuz H., Ozbey G., Cevik A., Tarakci B., Turgut-Balik D.

REVUE DE MEDECINE VETERINAIRE, cilt.157, ss.197-202, 2006 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 157 Konu: 4
  • Basım Tarihi: 2006
  • Dergi Adı: REVUE DE MEDECINE VETERINAIRE
  • Sayfa Sayısı: ss.197-202

Özet

The aims of this study were to analyse the direct pathogene role of Ornithobacterium rhinotracheale (ORT) in the respiratory tract of birds and to identify a set of techniques useful for infection diagnosis. For that, a total of 30 male 10 week old quails were infected via aerosol route by ORT strain B3263/91 (serotype A) and 30 other quails were used as negative controls. On the 1(st), 3(rd) and the 6(th) weeks post infection, 10 birds of each group were slaughtered, then lung and trachea were examined by bacteriological (cultures and PCR) and immuno-histochemical methods. In addition, the occurrence of serum anti-ORT antibodies was investigated by an enzyme-linked immunosorbent assay (ELISA).