OPTIMIZATION OF MEGAKARYOCYTE TRAPPING FOR PLATELET FORMATION IN MICROCHANNELS

Creative Commons License

Baydar Atak G., Insel M. A., Oruc M. E., Sadıkoğlu H.

CHEMICAL INDUSTRY & CHEMICAL ENGINEERING QUARTERLY, cilt.28, sa.1, ss.19-28, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 1
  • Basım Tarihi: 2022
  • Doi Numarası: 10.2298/ciceq201224012b
  • Dergi Adı: CHEMICAL INDUSTRY & CHEMICAL ENGINEERING QUARTERLY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.19-28
  • Anahtar Kelimeler: microfluidics, biotechnology, mathematical modeling, plate-let, COMSOL Multiphysics, IN-VITRO, EX-VIVO, GENERATION, BIOREACTOR, SEPARATION
  • Yıldız Teknik Üniversitesi Adresli: Evet

Özet

Platelets (PLTs) are responsible for stopping bleeding. They are small cell fragments produced from megakaryocytes (MKs) in the bone marrow. Low platelet count is a significant health problem for a patient. PLTs can usually be stored for up to 5 days prior to transfusion. Instantaneous production of PLTs from isolated and stored MKs is crucial for the patient's health. Thanks to microfluidic platforms, PLTs can be produced instantaneously from MKs. Herein, we have computationally studied fluid dynamics in the microchannels with slit structures and different inlet geometries. Analysis of the flow dynamics was performed by the commercial analysis software. The effects of flow rates and the angle between the inlet channels on the MKs trapping were investigated. The optimization of the angle between inlet channels and flow rates of main and pressure flows was done with response surface methodology (RSM) by counting the trapped MKs. The optimum conditions lead to the percentage of trapped MKs being 100 with a relative deviation of <1%. We also concluded that flow rates to trapping a higher amount of MKs are as important as the angle between the inlet channels.