Assessment of free-radical scavenging activity of Gypsophila pilulifera: assay-guided isolation of verbascoside as the main active component

Chima N. K., Nahar L., Majinda R. R., ÇELİK S., Sarker S. D.

REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, vol.24, no.1, pp.38-43, 2014 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 24 Issue: 1
  • Publication Date: 2014
  • Doi Number: 10.1590/0102-695x20142413391
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.38-43
  • Keywords: Gypsophila pilulifera, Free-radical scavenger, Solid-phase extraction, High-performance liquid chromatography (HPLC), Phenylethanoid, Verbascoside, AERIAL PARTS, PHENYLETHANOID GLYCOSIDES, CALCEOLARIA-TALCANA, OXIDATIVE STRESS, BIOACTIVITY, EXTRACTS, SEEDS, BIOASSAY, LIGNANS, PLANTS
  • Yıldız Technical University Affiliated: Yes


Gypsophila pilulifera, Boiss & Heldr, Caryophyllaceae, is a perennial medicinal herb that grows in the-southwestern region of Turkey. Except for only one report on the isolation-of cytotoxic. saponins from the underground parts of G. pilulifera, there are no published thorough phytochemical or bioactivity studies on this specie. In the present study; the free-radical Scavenging activity of extracts and fractions of the-stems of G. pilulifera was evaluated, using a. slightly modified and more-precise-version of the 2,2-diphenyl-1-picrylhydrazyl (DRPH) assay, :reported here for the first time. The DPPH assay-guided HPLC-PDA-purification of the active solid-phase extraction fraction (50% methanol in water) of the methanolic-extract exhibited verbascoside as the main free-radical Scavenger present in this species. The structure of this active compound was resolved by spectroscopy, and the free-radical scavenging potential of verbascoside was determined. (C) 2014 Sociedade Brasileira. de Farmacognosia. Published by Elsevier Editora Ltda. All rights reserved..