Application of l-asparaginase to produce high-quality Turkish coffee and the role of precursors in acrylamide formation

Akgun B., Arici M., Cavus F., Karatas A. B., Eksi Karaagac H., Ucurum H. O.

JOURNAL OF FOOD PROCESSING AND PRESERVATION, vol.45, no.6, 2021 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 45 Issue: 6
  • Publication Date: 2021
  • Doi Number: 10.1111/jfpp.15486
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, Business Source Elite, Business Source Premier, CAB Abstracts, Compendex, Food Science & Technology Abstracts, INSPEC, Veterinary Science Database
  • Yıldız Technical University Affiliated: Yes


In this study, the optimized conditions for l-asparaginase treatment on Arabica coffee beans and the impacts of two independent variables (enzyme dosage and treatment time) on five dependent variables (acrylamide, 2-Acetyl-3-Methylpyrazine, 2,3-Dimethylpyrazine, 2,5-Dimethylpyrazine, and 2-Methylpyrazine) were investigated by employing RSM. Green coffee beans were treated with l-asparaginase at various dosages (0-4000 ASNU/kg green bean) and times (30-120 min). Statistical analysis indicated that levels of responses were significantly related to enzyme dosage and treatment time (p < .05). These independent parameters had a significant influence on the quantity of acrylamide in Turkish coffee (p < .05), but enzyme dosage was found to be more effective (p < .01). The optimum conditions of l-asparaginase treatment for producing superior quality Turkish coffee were determined as 2,126.4 ASNU/kg green bean and 82.73 min. Also, a moderate positive correlation (r = 0.669, p < .01) was found between the asparagine concentration in green coffee beans and acrylamide content of coffees produced from these beans.