The effects of saponin on amylase and polyphenol oxidase (PPO) activities in embryo and endosperm tissues of wheat seeds during germination were investigated. Germination of wheat seeds was inhibited by saponin from beginning of germination up to 49 h. Amylase activity was found to be higher in endosperm than in embryo of wheat seeds, whereas it was contrary in the case of PPO activity. The effects of saponin on amylase and PPO activities were found to be different during seed germination. While saponin decreased amylase activity, it increased PPO activity. Amylase activity was lower in saponin-treated seeds than in control 0 up to 24 h of germination. Oil the other hand, PPO activity was higher in saponin treatment than in control between 0-12 h of germination time.