Each and every year, there has been an ever-increasing demand for organ transplants in geometric scales. There are no significant increase observed in organ donations while the need for organ transplantation is keep increasing. Organ donation emerges as the most feasible solution especially in heart valve diseases. Bioprostetic and mechanical valves have serious intrinsic problems and therfore they do not provide sufficent treatment for heart valve deficiencies. Due to these reasons, growing or fabricating tissue engineered heart valves using biomaterial scaffolds with a person's own stem cells with high biocompatability is an obligatory. The purpose of this study is; fabricating 3D artificial heart valves by stereolithography that has potential to be obtained for heart valve replacements. Stereolithography has proven potential for fabricating 3D models with high structural integrity and strong mechanical properties, yet it has not been evaluated as a bioprinter. In this study, this purpose is aimed to be succeeded and analyzed detailed biocompatibility characteristics of fabricated heart valves.