The simultaneous determination of Quinoline Yellow (E-104) and Sunset Yellow (E-110) in syrups and tablets by second derivative spectrophotometry

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ÖZGÜR M., KOYUNCU İ.

TURKISH JOURNAL OF CHEMISTRY, vol.26, no.4, pp.501-508, 2002 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 26 Issue: 4
  • Publication Date: 2002
  • Journal Name: TURKISH JOURNAL OF CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.501-508
  • Keywords: dyes, derivative spectrophotometry, pharmaceutical products, Sunset Yellow, Quinoline Yellow, PARTIAL LEAST-SQUARES, RATIO SPECTRA, MULTIVARIATE CALIBRATION, PONCEAU 4R, TARTRAZINE, SPECTROMETRY, COLORANTS, MIXTURES, DYES
  • Yıldız Technical University Affiliated: Yes

Abstract

A very simple spectrophotometric method using measurements at zero-crossing wavelength is described for resolving binary mixtures of the food dyes, Sunset Yellow (E-110) and Quinoline Yellow (E-104). Calibration graphs are linear up to 15.0 mugml(-1) of Sunset Yellow (r = 0.9998), and Quinoline Yellow (r = 0.9999). The assay procedure for E-110 and E-104 in pharmaceutical products involves the extraction of the dyes with AcOH/NaOAc buffer solution (pH = 4.5), filtration, and measurement of the second derivative absorbance values at 410.0 nm for E-104 and 533.1 nm for E-110. This method was used for determining synthetic mixtures of these dyes in different ratios and it was successfully applied to two commercial products without a previous separation step.