The simultaneous determination of Quinoline Yellow (E-104) and Sunset Yellow (E-110) in syrups and tablets by second derivative spectrophotometry


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ÖZGÜR M. , KOYUNCU İ.

TURKISH JOURNAL OF CHEMISTRY, cilt.26, ss.501-508, 2002 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 26 Konu: 4
  • Basım Tarihi: 2002
  • Dergi Adı: TURKISH JOURNAL OF CHEMISTRY
  • Sayfa Sayıları: ss.501-508

Özet

A very simple spectrophotometric method using measurements at zero-crossing wavelength is described for resolving binary mixtures of the food dyes, Sunset Yellow (E-110) and Quinoline Yellow (E-104). Calibration graphs are linear up to 15.0 mugml(-1) of Sunset Yellow (r = 0.9998), and Quinoline Yellow (r = 0.9999). The assay procedure for E-110 and E-104 in pharmaceutical products involves the extraction of the dyes with AcOH/NaOAc buffer solution (pH = 4.5), filtration, and measurement of the second derivative absorbance values at 410.0 nm for E-104 and 533.1 nm for E-110. This method was used for determining synthetic mixtures of these dyes in different ratios and it was successfully applied to two commercial products without a previous separation step.