The simultaneous determination of Quinoline Yellow (E-104) and Sunset Yellow (E-110) in syrups and tablets by second derivative spectrophotometry

Creative Commons License

ÖZGÜR M., KOYUNCU İ.

TURKISH JOURNAL OF CHEMISTRY, cilt.26, sa.4, ss.501-508, 2002 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 26 Sayı: 4
  • Basım Tarihi: 2002
  • Dergi Adı: TURKISH JOURNAL OF CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.501-508
  • Anahtar Kelimeler: dyes, derivative spectrophotometry, pharmaceutical products, Sunset Yellow, Quinoline Yellow, PARTIAL LEAST-SQUARES, RATIO SPECTRA, MULTIVARIATE CALIBRATION, PONCEAU 4R, TARTRAZINE, SPECTROMETRY, COLORANTS, MIXTURES, DYES
  • Yıldız Teknik Üniversitesi Adresli: Evet

Özet

A very simple spectrophotometric method using measurements at zero-crossing wavelength is described for resolving binary mixtures of the food dyes, Sunset Yellow (E-110) and Quinoline Yellow (E-104). Calibration graphs are linear up to 15.0 mugml(-1) of Sunset Yellow (r = 0.9998), and Quinoline Yellow (r = 0.9999). The assay procedure for E-110 and E-104 in pharmaceutical products involves the extraction of the dyes with AcOH/NaOAc buffer solution (pH = 4.5), filtration, and measurement of the second derivative absorbance values at 410.0 nm for E-104 and 533.1 nm for E-110. This method was used for determining synthetic mixtures of these dyes in different ratios and it was successfully applied to two commercial products without a previous separation step.