Electrochemical biosensing of cortisol in a hormone tablet and artificial bodily fluids

Olgaç N., Karakuş E., ŞAHİN Y., Liv L.

Diamond and Related Materials, vol.132, 2023 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 132
  • Publication Date: 2023
  • Doi Number: 10.1016/j.diamond.2022.109622
  • Journal Name: Diamond and Related Materials
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Chemical Abstracts Core, Communication Abstracts, INSPEC, Metadex, Civil Engineering Abstracts
  • Keywords: Cortisol, Anti-cortisol antibody, Biosensor, Artificial samples, Voltammetry
  • Yıldız Technical University Affiliated: Yes

Abstract

© 2022This article describes a new voltammetric biosensor developed using a glassy carbon electrode functionalised with an anti-cortisol antibody to measure cortisol in a hormone tablet and artificial bodily fluids containing saliva, urine and sweat. The anodic signal of the produced biosensor at −60 mV linearly increased with the amount of cortisol. Cyclic voltammetry, scanning electron microscopy, energy dispersive X-ray spectroscopy, UV–visible spectrophotometry and Fourier transform infrared spectroscopy were performed to characterise the biosensor. Among the results, the biosensor detected 2 pM of cortisol in a linear range from 0.025 nM to 5 μM. Moreover, the method demonstrated remarkable recovery values (99.90–102.06 %) with relative standard deviations of 1.88–3.82 % in real samples, which suggests its efficiency without being affected by the sample matrix.