Evaluation of The Effect of NCC on Phase Stability of HA-Based Injectable Bone Grafts

Hazar A. B. , Türkcan J. H.

International Congress on Vocational And Technical Science (UMTEB), Nakhchivan, Azerbaijan, 16 - 18 October 2020, pp.76

  • Publication Type: Conference Paper / Summary Text
  • City: Nakhchivan
  • Country: Azerbaijan
  • Page Numbers: pp.76


The injectable regenerative bone graft material (IRBGM), which reduces the risk of infection, prevents surgical scarring and intense blood loss, gains importance. The IRBGM consist of a biopolymeric carrier matrix with the CaP as bioactive phase. The stability of suspension during storage or application is the main issue to being "ready to use" since the biopolymer can promote the particle aggregation. Nowadays researchers focus on the phase separation mechanism and sedimentation of particles in terms of rheological behavior. To enhance the injectability without separation it is essential to increase the viscosity of the system [1, 2]. Studies have shown that biocompatible and bioactive Nanocrystalline Cellulose (NCC) with different surface charge densities affects the rheological behavior by affecting the particle-gel interactions. Also, it was reported that in aqueous conditions the NCC has relatively high viscosity which provides structural development. By using sulphuric acid for hydrolysis of cellulose negatively charged particles that is more stable in suspension due to electrostatic repulsion, can obtain [3, 4]. In the present study it was aimed to enhance the stability of the IRBGM by using the hyaluronic acid (HA) gel as carrier system with the addition of the NCC and the CaP as bioactive phase. Therefore, 0,1 wt%, 0,3 wt% and 0,5 wt% of the NCC was added into the carrier system. Citric acid (CA) which is a well-known stabilizing agent was also prepared in same proportion and used as a control group. Both the NCC and CA have unpaired -OH groups in their structure. As a biopolymer the NCC has more reactive groups compared to the CA. Thus, it is expected that the NCC can also stabilize the system. The stability of the suspensions was investigated with FTIR, Zeta Potential and Stability Tests. Finally, the effects of the addition of the NCC as potential stabilizing agent on phase stability was evaluated and compared with the CA.