Determination of total phenolic content and antidiabetic, antioxidant and antiproliferative activities of Gypsophila pilulifera extracts grown by in vitro culture

Ustuner H., NASIRCILAR A. G., Servi H., Demir Ü., ŞEN A., Gundogdu B., ...More

Biocatalysis and Agricultural Biotechnology, vol.56, 2024 (ESCI) identifier

  • Publication Type: Article / Article
  • Volume: 56
  • Publication Date: 2024
  • Doi Number: 10.1016/j.bcab.2023.103014
  • Journal Name: Biocatalysis and Agricultural Biotechnology
  • Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus, BIOSIS, Food Science & Technology Abstracts
  • Keywords: (3β,22E)-ergosta-7,22-dien-3-ol, (Z,Z)-9,12-octadecadienoic acid, Antiproliferative activity, Gypsophila pilulifera, In vitro culture, Palmitic acid
  • Yıldız Technical University Affiliated: Yes


This study aimed to evaluate the antidiabetic, antioxidant, anti-proliferative activities and total phenolic content of the methanol extracts of Gypsophila pilulifera produced in vitro. Root-A and Root-B showed strong anti-proliferative activity against PC3 and LNCaP cells lines. Root-A with IC50 value of 34.4 μg/mL and Root-B with IC50 value of 26.38 μg/mL in LNCaP cells displayed better anti-proliferative potential than Root-A with IC50 value of 54.18 μg/mL and Root-B with IC50 value of 52.07 μg/mL in PC3 cells. The highest total phenolic level has been detected in Branch-A which showed highest activity in ABTS test with an IC50 value of 96 μg/mL. All methanol extracts displayed poor antidiabetic activity. These results proved that Root-A and Root-B have significant antiproliferative activity. Root-A and Root-B extracts also showed differential effect in cancer cells compared to the normal cells. This study proposed the root extracts of Gypsophila pilulifera as a source of novel anti-cancer compounds for prostate cancer in the literature.