A novel, ecofriendly, and easy extraction and preconcentration method named as vortex-assisted spraying-based fine droplet formation liquid-phase microextraction was proposed for the determination of prochloraz at trace levels in orange juice samples by gas chromatography-mass spectrometry (GC-MS). In this novel system, extraction solvent is dispersed by the help of spraying apparatus instead of dispersive solvent. Various parameters of the method were carefully optimized to increase signal-to-noise ratio of the analyte. Under the optimum chromatographic and extraction conditions, limit of detection and limit of quantification were calculated as 3.2 and 10.8 mu g/kg, respectively. Moreover, enhancement in quantification power for the GC-MS system was determined as 372 folds based on LOQ comparison. Relative recovery results for orange juice samples were found to be between 95.0-107.7% by utilizing matrix matching calibration. Furthermore, the developed method may be used to efficiently and simply extract other organic compounds for their determinations in several matrices.