Microcapillary Culture Method: A Novel Tool for In Vitro Expansion of Stem Cells from Scarce Sources


ALLAHVERDİYEV A. , BAYDAR S. Y. , BAĞIROVA M. , FINDIKLI N.

ARCHIVES OF MEDICAL RESEARCH, cilt.43, ss.423-430, 2012

  • Cilt numarası: 43 Konu: 6
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1016/j.arcmed.2012.08.011
  • Dergi Adı: ARCHIVES OF MEDICAL RESEARCH
  • Sayfa Sayısı: ss.423-430

Özet

Background and Aims. Although increasing numbers of studies report the derivation of stem cells from a variety of different tissues, derivation efficiencies greatly vary among different studies even for the same tissue source. Hence, a consistent and efficient isolation protocol has not yet been established to date. Several factors have so far been documented that influence and limit mesenchymal stem cell (MSC) isolation and cultivation, including the age and gender of the tissue donor, origin of the tissue, amount of sampled tissue material and cell culture characteristics including the choice of basal media, serum, gas composition, etc. The aim of the study was to investigate the microcapillary culture method (MCM) to establish an efficient and consistent isolation as well as cultivation protocol by comparing the results with other classic culture systems (flasks, center wells).