Determination of homocystine as dabsyl derivative by HPLC using precolumn derivatization


Alpdogan G. , SUNGUR S.

ANALYTICAL LETTERS, vol.33, no.13, pp.2645-2651, 2000 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 13
  • Publication Date: 2000
  • Doi Number: 10.1080/00032710008543213
  • Title of Journal : ANALYTICAL LETTERS
  • Page Numbers: pp.2645-2651

Abstract

An HPLC method has been developed for the determination of homocystine. Homocystine was converted to its chromophoric dabsyl derivative and was chromatographed by a reversed phase chromatographic column (mu-Bondapak C) with pH = 3 phosphate buffer-acetonitrile (60:40) as isocratic mobile phase. The derivative was detected by a variable wavelength detector operating at 436 ran. A linear relationship was obtained between peak areas and homocystine concentrations over the range of 0.54-2.68 mu g / 20 mu l.