Determination of homocystine as dabsyl derivative by HPLC using precolumn derivatization
ANALYTICAL LETTERS, vol.33, no.13, pp.2645-2651, 2000 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 33 Issue: 13
- Publication Date: 2000
- Doi Number: 10.1080/00032710008543213
- Journal Name: ANALYTICAL LETTERS
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.2645-2651
- Yıldız Technical University Affiliated: Yes
Abstract
An HPLC method has been developed for the determination of homocystine. Homocystine was converted to its chromophoric dabsyl derivative and was chromatographed by a reversed phase chromatographic column (mu-Bondapak C) with pH = 3 phosphate buffer-acetonitrile (60:40) as isocratic mobile phase. The derivative was detected by a variable wavelength detector operating at 436 ran. A linear relationship was obtained between peak areas and homocystine concentrations over the range of 0.54-2.68 mu g / 20 mu l.