Analytical Letters, vol.10, no.40, pp.1893-1903, 2007 (SCI-Expanded)
Sunset Yellow (SY), Carmoisine (C), Ponceau 4R (P), and Patent Blue V (PB) are synthetic organic dyes which are under governmental regulations all over the world because of their toxicity and carcinogenicity. In this study, a simple and fast analytical procedure was proposed for the simultaneous determination of food dyes (SY, C, P, and PB) in powder drinks by means the partial least‐square treatment of spectrophotometric absorbance between 450 –730 nm, taken at 10 nm intervals. The experimental calibration matrix was constructed with 27 samples. The concentration ranges considered were 2, 3, 4 µg · ml−1 for SY, 7, 8, 9 µg · ml−1 for C, 9, 10, 11 µg · ml−1 for P, and 0.3, 0.4, 0.5 µg · ml−1 for PB. The method was applied to the determination of dyes in different commercially available powder drinks. The results obtained by the application of the PLS‐2 method were statistically compared with those obtained by an HPLC method using the F and t tests. Very similar values were found by two methods. No time consuming pretreatment was needed and this method also provides rapid, accurate and economical analysis of these colors.