Akademik Veri Yönetim Sistemi
5th International Eurasian Conference on Biological and Chemical Sciences, Ankara, Türkiye, 23 - 25 Kasım 2022, ss.344
Hemp seed oil is a nutritious oil containing over 80% polyunsaturated fatty acids obtained by cold pressing from the seeds
of the Cannabis Sativa L. plant. It contains omega-6 fatty acids such as linoleic acid (LA, 54%), gamma-linolenic acid
(GLA, 3%) and omega-3 fatty acids (alpha-linoleic acid (ALA, 17%) and stearidonic acid (2%). The hemp seed oil shows
pharmacological activity due to the benefits it provides. The antibacterial property of the oil is proven against gramnegative
bacteria Escherichia coli (E.Coli) and gram-positive bacteria Staphylococcus auerus (S.Aureus) according to
the disc diffusion method. Due to this antibacterial feature, hemp seed oil is expected to provide an antibacterial wound
healing environment and accelerate healing thanks to the components it contains. Therefore, in the present study, it was
aimed to investigate the use of hemp seed oil in a wound dressing system. For this purpose, it was tried to obtain a wound
dressing system with nanofiber structure with the most suitable fiber diameter by encapsulating hemp seed oil into
gelatin/sodium alginate nanofibers by electrospinning method. The antibacterial activity test results showed that the
antibacterial activity decreased with the decrease in the oil concentration. Furthermore, three-level, three-variable Box-
Behnken design-based response surface methodology was employed as an optimization tool to select the most appropriate
parameter settings required to obtain the desired fiber diameter. Accordingly, the diameter of the nanofibers obtained
from the solutions prepared at different component concentrations based on the experimental design was investigated by
using scanning electron microscopy (SEM) analysis and a second-order polynomial equation was developed to estimate
the fiber diameter. It was found that the experimental data and the developed models were in good agreement. The
effectiveness of the nanofiber wound dressing material fabricated under optimum conditions based on the design results
will be supported by future characterization tests.