Cumulus Cells Are Potential Candidates for Cell Therapy


Mohammed E. E. , Yılmaz S., Akcin O. A. , Nalbantoğlu B. , Fıcıcıoglu C., Sahin F., ...More

IN VIVO, vol.33, no.6, pp.1921-1927, 2019 (Journal Indexed in SCI) identifier identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 6
  • Publication Date: 2019
  • Doi Number: 10.21873/invivo.11686
  • Title of Journal : IN VIVO
  • Page Numbers: pp.1921-1927

Abstract

Background/Aim: Cumulus cells (CCs) originate from the membrane granulosa cells and surround oocytes during follicle maturation. CCs produce high levels of hyaluronan that targets CD44, which is a major tumorigenic marker. This study aimed to investigate whether CCs have a role in cell therapy by targeting CD44 in pancreatic cancer cells. Materials and Methods: CCs were isolated from the oocytes and incubated in a hypoxic environment. BxPC-3 pancreatic cancer cells were treated with CC conditioned media for three days. Results: Conditioned media of CC cells incubated in hypoxic conditions caused a 25% reduction in the viability of BxPC-3 cells. Expression of anti-apoptotic genes was down-regulated, while that of pro-apoptotic genes was upregulated. An increased number of BxPC-3 cells exhibited increased levels of reactive oxygen species and arrested in the synthesis (S) phase of the cell cycle. Conclusion: CCs conditioned medium induced apoptosis of pancreatic cancer cells.