Akademik Veri Yönetim Sistemi
Bulgarian Chemical Communications, cilt.57, sa.3, ss.202-205, 2025 (Scopus)
In this study, it was aimed to develop a new high-performance liquid chromatography (HPLC) method for the efficient extraction and quantification of coumarin compound from Helichrysum arenarium, Origanum onites and Achillea millefolium plants by Soxhlet extraction method. Deionized water, methanol, ethanol, acetone, n-hexane and toluene were used as solvents in Soxhlet extraction and the most suitable solvent was determined by comparing the extraction efficiencies of the solvents. Experimental results revealed that there were significant differences in coumarin extraction efficiency depending on solvent type and chemical structure of the plant. Deionized water provided the highest extraction efficiency in general, while the highest coumarin concentration (0.0339 mg/mL) was obtained for Helichrysum arenarium when methanol was used. Achillea millefolium was found to have the lowest overall coumarin content. The HPLC method developed within the scope of the study provided high sensitivity, accuracy and reproducibility for coumarin quantification. The findings indicate that the choice of solvent for the analysis of coumarin-containing herbal extracts should not be based solely on polarity, but should be evaluated together with the matrix properties of the plant.