Synthesis of Isotopic Labelled Paracetamol Benzoate-d3 and Its Application in the Determination of Paracetamol

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Demirel R., ERARPAT BODUR S., Bodur S., GÜNKARA Ö. T., BAKIRDERE S., TURAK F.

ChemistrySelect, cilt.8, sa.13, 2023 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 8 Sayı: 13
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1002/slct.202203851
  • Dergi Adı: ChemistrySelect
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier
  • Anahtar Kelimeler: Benzoyl chloride, determination, GC-MS, isotope dilution, paracetamol
  • Yıldız Teknik Üniversitesi Adresli: Evet

Özet

This study proposed a precise/accurate analytical method for the paracetamol (PCT) determination in human serum/urine matrices. PCT was simultaneously derivatized and preconcentrated by dispersive liquid-liquid microextraction (DLLME). Benzoyl chloride dissolved in dichloromethane was used as derivatization agent in the extraction solvent. Quadruple isotope dilution (QID) method with three calibration mixtures (A*B-x, x=1,2,3) and one sample mixture (AB) was performed to enhance the method accuracy and precision. In our research laboratory, paracetamol benzoate-d3 (PCT benzoate-d3) was synthesized and used as isotopically labelled substance (B) to conduct QID method. After the combination of QID and DLLME-GC-MS systems, percent recovery results for human serum/urine matrices were found to be 99.3–101.0 % and 98.8–99.9 %, respectively.