An accomplished procedure of horseradish peroxidase immobilization for removal of acid yellow 11 in aqueous solutions

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WATER SCIENCE AND TECHNOLOGY, vol.81, no.12, pp.2664-2673, 2020 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 81 Issue: 12
  • Publication Date: 2020
  • Doi Number: 10.2166/wst.2020.326
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Agricultural & Environmental Science Database, Analytical Abstracts, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chimica, Compendex, EMBASE, Environment Index, Geobase, MEDLINE, Pollution Abstracts, Public Affairs Index, Veterinary Science Database
  • Page Numbers: pp.2664-2673
  • Keywords: alginate entrapment, azo dye removal in aquatic solutions, decolorization, enzyme immobilization, glutaraldehyde crosslinking, horseradish peroxidase, FUNCTIONAL-PROPERTIES, AZO-DYE, DECOLORIZATION, STABILITY, IMPROVEMENT, LACCASE, ENZYME, PHENOL
  • Yıldız Technical University Affiliated: Yes


Horseradish peroxidase (HRP) characteristics were improved by two techniques, Na-alginate entrapment and glutaraldehyde crosslinking prior to alginate entrapment, in order to enhance the stability, functionality and removal of dyes in waste water. Free, entrapped and crosslinked-entrapped enzymes were compared by activity assays, which indicated the optimum temperature is 25 degrees C and pH 4.0-5.0. Kinetics results showed that alginate entrapment and crosslinking prior to entrapment increased V(max)and did not cause any significant decrease in K-m. The thermal resistance of the free enzyme was short-term, zero residual activity after 250 min, while the immobilized enzymes preserved more than 50% of their activity for 5 h at 60 degrees C. Immobilized HRP was resistant to methanol, ethanol, DMSO and THF. The storage stability of free HRP ended in 35 days whereas entrapped and crosslinked-entrapped HRPs had 87 and 92% residual activity at the 60th day, respectively. HRP was used in the decolorization of azo dye Acid yellow 11 and total decolorization (>99%) was obtained using crosslinked-entrapped HRP. Reusability studies presented the improvement that crosslinked-entrapped HRP reached 74% decolorization after 10 batches. The results demonstrated that the novel immobilized HRP can be used as an effective catalyst for dye degradation of industrial waste effluents.