The aim of this study is to make simulations with Matlab/Simulink to be used in the production phase of the autoclave in the sterilization unit. Sterilization unit is very important in terms of hospital employees and patients for protecting from infections and accidents. The study examined autoclave equipments that are fabricated based on the EN standards, then mathematical calculations were performed by (1)the references obtained from these calculations. These references transferred as the necessary characteristics for MATLAB/Simulink modelling. By analyzing these characteristics, the impact of the variations throughout the production or development pahese can be predicted by investigating the changes in these characteristics. After this study, it is anticipated to convet this model into prototypes and obtain more tangible result. In this study, embedded sytems are used and the data is transferred to an electronic media. Later, the system will be tested on one of the autoclaves that is currently available on the market. It is planned that by exchanging the mainboard of the autoclave with another mainbored which the new software has installed and inspected on the device, afterwards.