Akademik Veri Yönetim Sistemi
3rd International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2020) , Ankara, Türkiye, 19 - 20 Mart 2020, ss.767
INVESTIGATION
OF ANTIOXIDANT ACTIVITY OF CATECHIN LOADED NANOPARTICLES
Burcu TURKOGLU*1, Doç. Dr. Banu MANSUROGLU1
1 Yildiz Technical University, Faculty of Science and Letters,
Department of Molecular Biology and Genetics,
34210 Esenler, Istanbul, TURKEY
*Corresponding author e-mail: burcutuncerr@gmail.com
Abstract
The aim of the present study was to investigate the synthesis,
characterization and biological activity of Catechin loaded PLGA nanaoparticles(CatNP).
CatNP with PLGA copolymer with poly-lactic acid / poly-glycolic acid (PLA /
PGA) (50:50) compositions were prepared by a single emulsion-solvent-evaporation
method. Encapsulation efficiencies of the CatNP calculated from the upper phase
indirect assay method using UV spectrophotometer measurements. Release study of
active substance from PLGA nanoparticles at different pH media was continued
for 45 days and CatNP was found to release 88% with the highest release amount
in pH 6 environment. It has been observed that PLGA nanoparticles were
successfully loaded by SEM (Scanning Electron Microscopy), AFM (Atomic Force
Microscopy) and FTIR (Fourier Transform Infrared Spectroscopy) analyzes. The
biological activity of comparatively encapsulated and free form of Catechin was investigated. The antioxidant activity of
the CatNP was investigated by the 2.2-Diphenyl-1-picrylhydrazyl (DPPH) method.
Anticytotoxicity activity was demonstrated using MTT (3-
[4,5-dimethylthiazol-2-yl] -2,5-diphenyltetrazolium bromide) method on MCF7
breast cancer and C6 Glioma brain cancer
cell line. According to the free form of Cat, the antioxidant activity of CatNP
was observed for 45 days in all pH conditions, whereas the free form lost its
antioxidant activity nearly at the end of 15th day. Analysis of anticytotoxicity
activity on MCF7 and C6 Glioma cell lines revealed that the encapsulated
Catechin had anticytotoxicity activity on the MCF7 cell line for 72 hours while
the its effect on C6 glioma cell line has been shown to have a reduced effect
of anticytotoxicity activity for up to 48 hours. The results show that the PLGA
nanoparticle system provides controlled and long-term release of Catechin. In
addition, it has been determined that PLGA nanoparticular system increases the
biocompatibility and bioavailability of Catechin by protecting from
biodegradation and environmental conditions.
Keywords: Catechin, Polyphenolic compunds, Nanoparticle, Poly(D,L-lactic-co-glicolic acid), Antioxidant Activity, Anticytotoxicity Activity
Acknowledgements: This work was supported by the Yildiz
Technical University Scientific Research Projects Coordination Department,
project numbers 2015-01-07-YL04