Investigation Of Biological Activity Of Catechin Loaded Nanoparticles


Mansuroğlu B. , Türkoğlu B.

3rd International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2020) , Ankara, Türkiye, 19 - 20 Mart 2020, ss.767

  • Basıldığı Şehir: Ankara
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.767

Özet

INVESTIGATION OF ANTIOXIDANT ACTIVITY OF CATECHIN LOADED NANOPARTICLES

 

Burcu TURKOGLU*1, Doç. Dr. Banu MANSUROGLU1

 

1 Yildiz Technical University,  Faculty of Science and Letters,

Department of Molecular Biology and Genetics, 34210  Esenler, Istanbul, TURKEY

 

*Corresponding author e-mail: burcutuncerr@gmail.com

 

Abstract

The aim of the present study was to investigate the synthesis, characterization and biological activity of Catechin loaded PLGA nanaoparticles(CatNP). CatNP with PLGA copolymer with poly-lactic acid / poly-glycolic acid (PLA / PGA) (50:50) compositions were prepared by a single emulsion-solvent-evaporation method. Encapsulation efficiencies of the CatNP calculated from the upper phase indirect assay method using UV spectrophotometer measurements. Release study of active substance from PLGA nanoparticles at different pH media was continued for 45 days and CatNP was found to release 88% with the highest release amount in pH 6 environment. It has been observed that PLGA nanoparticles were successfully loaded by SEM (Scanning Electron Microscopy), AFM (Atomic Force Microscopy) and FTIR (Fourier Transform Infrared Spectroscopy) analyzes. The biological activity of comparatively encapsulated  and free form of Catechin  was investigated. The antioxidant activity of the CatNP was investigated by the 2.2-Diphenyl-1-picrylhydrazyl (DPPH) method. Anticytotoxicity activity was demonstrated using MTT (3- [4,5-dimethylthiazol-2-yl] -2,5-diphenyltetrazolium bromide) method on MCF7 breast cancer  and C6 Glioma brain cancer cell line. According to the free form of Cat, the antioxidant activity of CatNP was observed for 45 days in all pH conditions, whereas the free form lost its antioxidant activity nearly at the end of 15th day. Analysis of anticytotoxicity activity on MCF7 and C6 Glioma cell lines revealed that the encapsulated Catechin had anticytotoxicity activity on the MCF7 cell line for 72 hours while the its effect on C6 glioma cell line has been shown to have a reduced effect of anticytotoxicity activity for up to 48 hours. The results show that the PLGA nanoparticle system provides controlled and long-term release of Catechin. In addition, it has been determined that PLGA nanoparticular system increases the biocompatibility and bioavailability of Catechin by protecting from biodegradation and environmental conditions.

 

Keywords: Catechin, Polyphenolic compunds, Nanoparticle, Poly(D,L-lactic-co-glicolic acid), Antioxidant Activity, Anticytotoxicity Activity

Acknowledgements: This work was supported by the Yildiz Technical University Scientific Research Projects Coordination Department, project numbers 2015-01-07-YL04