A GENERAL OVERVIEW: PRODUCTION OF PLATELETS IN A MICROCHANNEL


Baydar Atak G. , Oruç M. E. , Sadıkoğlu H.

5TH INTERNATIONAL CONFERENCE ON ADVANCES IN MECHANICAL ENGINEERING , İstanbul, Türkiye, 17 - 19 Aralık 2019, ss.1204

  • Basıldığı Şehir: İstanbul
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayısı: ss.1204

Özet

ABSTRACT

Platelets are component of blood and they are responsible for stopping the bleeding. Platelets play an 

important role in health problems such as thrombosis, cancer, inflammation etc. They are small cells that are derived 

from bigger cells called megakaryocytes. Platelets cannot be stored because of their 5-day shelf life and also a possible 

bacterial contamination. In daily life in case of need, platelets are provided by living donors. More than 2.17 million 

apheresis equivalent platelet units are transfused yearly in the US at a cost of >$1 billion per year. In recent years, 

various groups have either succeeded in producing megakaryocytes that produce platelets. Thanks to microfluidics, 

platelets can be produced instantaneously from megakaryocytes that isolated and stored before. But these studies have 

suffered from same limitations; first one is low yield of process and second one is artificial platelets cannot be replaced 

with physiological platelet products. To cope with these limitations, flow behaviors in microchannels should be 

observed correctly and operation conditions optimized maximized for the yield of process.

In microfluidic devices, liquid flowing in a microchannel behaves as in a laminar flow regime. Due to the 

laminar flow in the channels, the flow rate can be precisely controlled so that the well-defined shear stress can be 

applied for breaking megakaryocytes into platelets. In this study, we summarized the general limitations and operation 

conditions of platelet production in a microchannel. In accordance with the data in the literature, we offer optimum 

operation conditions and solutions for general problems in microchannels for platelet formation from megakaryocytes.

Keywords: Microfluidics, Biotechnology, Platelet, Megakaryocyte

ACKNOWLEDGEMENTS: This work was supported by Research Fund of the Yildiz Technical University. 

Project number FCD-2018-3150.