© 2018, Turkish Chemical Society. All rights reserved.Coriandrum sativum L. (coriander) oil has antibacterial, antifungal, and antimicrobial activity. This study prepared and characterized chitosan nanoparticles loaded with the oil from Coriandrum sativum L., using an ionic gelation method with tripolyphosphate as the cross-linker. The coriander oil-loaded chitosan nanoparticles were characterized by Zeta Sizer measurements, Fourier transform infrared spectrometry, scanning electron microscopy (SEM), electron dispersive X-ray spectroscopy (EDS), and in vitro release properties. It was shown that chitosan nanoparticles loaded with coriander oil have an average size of 113.5 nm, zeta potential of 16 mV and a polydispersity index of 0.378. The encapsulation efficiency was calculated as 72%. The spherical morphology of the nanoparticles was verified by SEM analysis, and EDS revealed the elemental composition. Furthermore, cytotoxicity analysis using L929 fibroblast cells and the XTT method demonstrated that coriander oil-loaded chitosan nanoparticles, coriander oil, and empty chitosan nanoparticles had no significant cell toxicity.