BAGY2 Retrotransposon Analyses in Barley Calli Cultures and Regenerated Plantlets

YILMAZ S., Marakli S., Gozukirmizi N.

BIOCHEMICAL GENETICS, cilt.52, sa.5-6, ss.233-244, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 52 Sayı: 5-6
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1007/s10528-014-9643-z
  • Dergi Adı: BIOCHEMICAL GENETICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.233-244
  • Anahtar Kelimeler: Hordeum vulgare, Somaclonal variation, BAGY2, Polymorphism, Copy number variations, SOMACLONAL VARIATION, MARKERS, GENOME, IRAP, DNA, ACTIVATION, MOVEMENTS, ELEMENTS, SYSTEM, REMAP
  • Yıldız Teknik Üniversitesi Adresli: Hayır

Özet

The stability of aging barley calli and regenerated plantlets from those calli was investigated by the BAGY2 retrotransposon-specific IRAP technique. Mature embryos of barley (Hordeum vulgare cv. Golden Promise) were cultured in Murashige and Skoog medium supplemented with 4 mg/L dicamba and maintained on the same medium for 45 and 90 days. Two IRAP-based primers were used, and the levels of variation of DNA isolated from 45- and 90-day-old calli and regenerated plantlets were found to be increased 0-21%, depending on the mature embryo material and the age of the callus. It has been observed that culture conditions cause genetic variations and evident BAGY2 retrotransposon alterations. Internal domains of BAGY2 were also analyzed by qPCR, and copy numbers were found to be increased. These findings are expected to contribute to understanding of how retrotransposons affect features like tissue culture (especially callus tissue) formation and genetic engineering studies.